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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which ends up in chromosome instability, and therefore may well lead to tumorigenesis. This protein was identified to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a critical function in selling mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two further splice variants were claimed but thorough nucleotide sequence information and facts isn't nevertheless readily available.
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Blocking peptides are peptides that bind specially to your target antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.
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This guidebook outlines even more attainable optimization of cellular and immunoassay parameters to be certain the absolute best success are...
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This tutorial outlines further attainable optimization of cellular and immunoassay parameters to be sure the absolute best success are...
Antibodies certain to the blocking peptide no more bind to your epitope on the concentrate on protein. By comparing the staining through the blocked antibody vs . the antibody on your own, you can see which staining is distinct.
Concentrate on Details Cyclin E belongs to the really conserved cyclin family members, whose associates are characterized by a extraordinary periodicity in protein abundance throughout the mobile cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins show distinctive expression and degradation styles which add to the temporal coordination of each and every mitotic party. Cyclin E varieties a fancy with and features for a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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